What is the classification of methanobrevibacter smithii.
We successfully cultured M. smithii from a liquid culture medium inoculated with stool collected from a healthy donor in an anaerobic chamber. The isolation in pure culture permitted successful culture on agar medium by our performing a co-culture with B. thetaiotaomicron. We also successfully tested the co-cultivation of M. smithii with other known hydrogen-producing bacteria.
In mice, increased prevalence of Methanobrevibacter smithii, a methanogenic bacteria species, is associated with increased weight gain. M smithii produces methane as a byproduct of hydrogen-requiring anaerobic metabolism. Methane produced in the intestine is expelled from the body by exhalation and flatus.
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The methanoarchaea Methanosphaera stadtmanae and Methanobrevibacter smithii are known to be part of the indigenous human gut microbiota. Although the immunomodulatory effects of bacterial gut commensals have been studied extensively in the last decade, the impact of methanoarchaea in human's health and disease was rarely examined. Consequently, we studied and report here on the effects of M.
Archaebacteria are primitive, single-celled microorganisms that are prokaryotes with no cell nucleus.Each archaea has the ability to live in very severe environments. Archaebacteria are one of the six kingdoms of life: plants, animals, protists, fungi, eubacteria and archaebacteria. Crenarchaeota Examples.
Domain: Archaea Kingdom: Euryarchaeota Phylum: Euryarchaeota Class: Methanobacteria Order: Methanobacteriales Family: Methanobacteriaceae Genus: Methanobrevibacter.
Methanobrevibacter smithii is the dominant methanogen isolated from the human colon (35, 36). Isolation of M. smithii involves the plating of fecal samples on antibiotic-containing medium and analyzing the incubating cultures for methane production ( 39 ).